Mycobacterium avium

By Karim Al-Itani and Yasamin Nassimi


The Mycobacterium avium complex (MAC) is composed of multiple nontuberculous mycobacterial species (NTM); this complex primarily consists of M.avium and Mycobacterium intracellulare. M.avium, is a slow-growing Gram-positive bacteria that is commonly found in soil and water sources. This intracellular pathogen has the ability to resist the immune mechanisms of the host, and the respiratory tract is amongst the most susceptible sites of infection. In addition to the infections within the respiratory tract, M.avium can also invade the bones, joints, lymph nodes and spread systemically. Infection acquisition may occur through ingestion, inhalation and dermal contact with environmental sources or medical devices. 


M. avium is an opportunistic pathogen, meaning it can cause disease when the host’s immune system is impaired (e.g. HIV). Acquisition of M. avium results in the adhesion of the microorganism to the mucosal epithelial cells in the intestinal lining and infection of the macrophages. Upon the invasion of the submucosal tissue and lymph nodes, the bacterium will be carried to the rest of the body via the lymphatics. Additionally, another risk factor for MAC infections includes the presence of underlying lung diseases. 

There are two major phenotypes associated with MAC pulmonary diseases (MAC-PD): fibrocavitary and nodular bronchiectatic (NB). The fibrocavitary form is characterized by the presence of lesions in the upper lobes of the lung, and occurs predominantly in older males with underlying lung diseases, such as previous pulmonary tuberculosis and chronic obstructive pulmonary disease (COPD). The NB form often develps in postmenopausal women and can cause multiple nodules in the right middle lobe and left upper lobe of the lung. 

Symptoms in patients with competent immune systems are nonspecific, with chronic cough being the most common symptom. Some patients with MAC-PD may also experience fever and hemoptysis, which is defined as coughing up blood or bloody mucus. 

Figure 1: Image of M. avium pulmonary disease of the NB form in a 47 year old Woman. Chest radiograph (2D X-ray imaging) shows small nodular clusters in both lower lung zones. High-Resolution Computed Topography (HRCT) (3D X-ray imaging) shows small centrilobular nodules in the right middle lobe and the left upper lobe. Source: Park, J. S. (2009). Respiratory Review of 2009: Nontuberculous Mycobacterium. Tuberculosis and Respiratory Diseases. The Korean Academy of Tuberculosis and Respiratory Diseases.


Currently, MAC species, especially M.avium, are mainly responsible for the majority of pulmonary infections caused by NTM. A Japanese study found that the incidence of MAC infections increased from 5.2/100,000 in 2007 to 13.1/100,000 in 2014; with the pulmonary diseases caused by M.avium having the highest prevalence in the north and east of Japan.

Comparably, in Europe, M.avium was the most frequent MAC species among pulmonary and extrapulmonary samples; as observed in studies done in Portugal (58.0%), Denmark (50.7%) and Italy (41.5%). 

The same overall pattern of MAC pulmonary and extrapulmonary diseases has been found in the United States of America, with M.avium being the most frequent isolate among MAC species (54%).

A potential explanation for the elevated prevalence of MAC and M.avium infections could be linked to the improvement of laboratory screening methods and increased awareness towards these infections in clinical settings. 

Virulence factor

M. avium bacteria are characterized by the presence of a water-repellent cell wall and by their mycolic acid constituents composing their core. Mycolic acids are densely packed with a variety of GPLs, which are a class of lipid and carbohydrate molecules linked together. The presence of GPLs is responsible for the immunomodulatory properties of M. avium that makes it a successful pathogen. 

First of all, the GPL component of the bacterium prevents the formation of the phagosome-lysosome complex. A phagosome is a cytoplasmic structure that exists inside specialized immune cells known as phagocytes; later, the fusion of the phagosome with the lysosome results in a phagolysosome complex. Formation of the phagolysosome complex is crucial for the destruction of intracellular microorganisms. The ability of GPLs to support the survival of mycobacteria in phagocytes is due to the interaction between the GPLs with specific receptors on the surface of the phagocytes. Moreover, the accumulation of GPLs inside macrophages leads to an amplified proinflammatory response. This phenomenon is essential as it leads to amplified infection impacts, without the necessary presence of the microorganism. 

Lastly, the synthesis of GPLs is required for the adhesion and secretion of biofilms. It has been suggested that inactivation of genes responsible for the synthesis of GPLs, leads to the impairment of the microorganism to slide or stick to surfaces. Thus, the presence of GPLs facilitates the microorganism to create a tight attachment with the host. Moreover, biofilm formation contributes to the adaptation of virulent strains to environmental anoxia and nutrient limitations, by lowering the metabolic rate and reducing the rate of cell division. 


Treatment strategy for MAC-PD depends on how far the disease has  progressed. In nearly half of the patients, untreated MAC-PD does not progress for several years after diagnosis and negative culture conversions (no bacteria detected by testing) are recorded. So, it is important to consider what risk factors patients have prior to the initiation of treatments to avoid unnecessary medical expenses and adverse drug reactions. Some risk factors include cavitary lesions, low BMI, poor nutritional status, and extensive disease symptoms. In cases where treatment is warranted, a three-drug combination of macrolide-based antibiotics is used. Standard treatment uses three doses per week with increased administrations in severe cases.

This approach has been associated with better patient outcomes. However, this was only recorded in 32-65% of patients based on 5 systematic reviews. On top of its limited success, this treatment plan is limited by macrolide-resistant MAC strains which do not have any effective medication options. In cases where drug treatment fails, lung resection surgery is considered. This has led to successful culture conversion rates between 80-100%, which refers to bacterial ability to go from being detectable to non-detectable. However, the surgical approach is associated with postoperative morbidity and mortality in 0 to 50% of cases. This number can be significantly lowered through improved patient selection, preoperative assessments, surgical techniques and postoperative measures. Finally, alternative therapeutic agents are being tested for macrolide-resistant MAC-PD. Clofazimine led to culture conversions in 87% and 99%. of patients in two studies conducted. However, adverse reactions have been recorded in roughly 50% of patients. Overall, the current treatment for MAC-PD is subpar and novel treatment approaches should be developed for patients with poor prognosis, especially those diagnosed with macrolide-resistant MAC. 

Figure 2: 68-year-old woman with productive cough hemoptysis (spitting blood) lasting for weeks. She recorded positive culture conversions for MAC prior to therapy. Images A and B are pre-therapy and C and D are post-therapy. (A) Chest computed tomography (CT) (3D X-ray imaging)  reveals small nodules, lesions, and mild bronchiectasis (bronchi walls thick due to inflammation) in the left upper lung. (B) Plain chest radiograph (2D X-ray imaging) indicates fluid build up in the left upper lung. (C) Chest CT showing the use of clarithromycin, ethambutol, and rifampin three-times-weekly for 7 months leads to reduced lesions in the lungs. (D) Chest radiograph shows improved left upper lung with less fluid build up. After treatment she recorded negative culture conversions for MAC. Source: Pan, S.-W., Shu, C.-C., Feng, J.-Y., & Su, W.-J. (2020). Treatment for mycobacterium avium complex lung disease. Journal of the Formosan Medical Association, 119.


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Akram, S. M., & Attia, F. N. (2020). Mycobacterium Avium Intracellulare. PubMed; StatPearls Publishing.

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Pan, S.-W., Shu, C.-C., Feng, J.-Y., & Su, W.-J. (2020). Treatment for mycobacterium avium complex lung disease. Journal of the Formosan Medical Association, 119.

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