Klebsiella pneumoniae

by Wenlie Xie and Huixuan Jia


Klebsiella pneumoniae was first described by a German microbiologist and pathologist Carl Friedländer in 1882. K. pneumoniae is found in the normal flora of skin, mouth and intestines, where it does not cause disease. Under opportunistic conditions such as a weakened host immune system or an altered microbiota, K. pneumoniae enters the hosts and causes several disease including pneumoniae, bloodstream infection and wound infection (Figure 1).

Figure 1: A photomicrographic view of a Hiss capsule-stained culture specimen revealing the presence of numerous Klebsiella pneumoniae bacteria. Source: https://phil.cdc.gov/Details.aspx?pid=14342.


Klebsiella infections are mainly acquired nosocomially, meaning that they are mostly encountered in hospitals or healthcare setting. Hospitalized patients with compromised immune systems are more susceptible: after two weeks of hospital admission, K. pneumoniae colonization in the patient’s intestine can increase up to two- to four-fold. This is mainly due to antibiotic medications that the patients are receiving, especially broad-spectrum or multiple antibiotics. Since the normal commensal micro-organic environment is damaged, this creates a favorable niche for K. pneumoniae colonies to grow. According to the Center of Disease Control and Prevention (CDC) of the USA, healthy people need not to worry about Klebsiella infections.

Under healthcare settings, K. pneumoniae is transmitted between individuals from contaminated invasive medical equipments, such as ventilators (breathing machines) or intravenous (vein) catheters. When K. pneumoniae reaches tissues or organs, it causes diseases including septicemia (blood poisoning), pneumonia, urinary tract infection (UTI), and soft tissue infection. Symptoms of infections vary from different infection sites. For example, hospital-acquired K. pneumoniae induces clinical symptoms such as fever and chills, shortness of breath, decreased blood pressure and faster heart rate, nausea and vomiting, etc. If the bacteria is introduced into the bloodstream, patients can develop symptoms such as sharp headache, nausea, dizziness and impaired memory.


Canada has only reported sporadic cases and limited outbreaks in hospitals from Ottawa and Montreal. In Europe, K. pneumoniae is reported as the second most common cause of bloodstream infections in adult population. Middle-aged and older people with debilitating diseases, such as diabetes mellitus are more susceptible to the infection. The ability of K. pneumoniae to spread rapidly also can lead to hospital outbreaks in neonatal units. The mortality rate is typically around 100% for patients with alcoholism as alcoholic patients suffer from weakened immune systems, which can increase their susceptibilities to tissue-damaging pathogens.

One noticeable outbreak was the 2011 Montreal Jewish General Hospital outbreak caused by a highly drug-resistant strain called KPC-producing K. pneumoniae. In this outbreak, 27 patients were identified to be infected and among them, 1 patient died from the infection. The outbreak shows that new antibiotic-resistant strains of K. pneumoniae are appearing. Antibiotic resistance is a huge problem because it leads to higher medical costs, prolonged hospital stays, and increased mortality rate. Feces are the most significant source of patient infection, followed by contact with contaminated medical equipment.

Virulence system

The most important virulence factor contributing the pathogenesis of K. pneumoniae is their ability to form a thick layer of biofilm, which consists of a large number of bacteria embedded in an extracellular matrix. Majority of the K. pneumoniae strains are biofilm-producing. The biofilm greatly enhances the bacteria’s ability to attach to abiotic environment such as medical equipments, and living organisms such as tissues. Under the healthcare setting, in order to invade human, K. pneumoniae uses pili (hair-like appendages for attachment) and capsular polysaccharides to attach on the surface of intrusive medical equipments, e.g. the catheter of a patient. This initial attachment allows the growth and colonization of the bacteria in an extracellular matrix on the surface of the catheter. At this stage, the exponentially-growed bacteria is sessile and might not be pathogenic. When the biofilm is matured and the sessile K. pneumoniae bacteria differentiates into planktonic form, K. pneumoniae sheds and free to attach to tissues or enter the bloodstream, causing infections. The biofilm-producing strains of K. pneumoniae often contributes to recurrent infections, since antibiotics only clear the infections within the body but not on the biofilm source.

Upon entering the body, K. pneumoniae uses lipopolysaccharides (LPS) and capsule against the body’s front-line immune defense, such as the complement system. Complement system is one of the host’s lines of defence, consisting of over 30 proteins. LPSs are long polysaccharide filaments on the bacterial outer membrane, which could trigger the activation of the complement system. A proposed mechanism is that LPS is masking under the capsule polysaccharides, of which the surface structure does not activate host complement system (Figure 2a). The longest O-polysaccharide chain from the LPS reaches the exterior milieu and preferentially fixes C3b, a component from complement system. This causes the deposition of C3b onto LPS molecules at distant sites from the bacterial cell membrane (Figure 2b). This inhibits the attachment of C3b to the surface thus the assembly of membrane attack complex is prevented (Figure 2c).

Figure 2: How K. pneumoniae uses LPS and capsule to avoid the host’s complement system. See text for details. Source: Wenlie Xie and Huixuan Jia.


K. pneumoniae infection can be treated with antibiotics if the strain is not drug-resistant. The best choices of antibiotics are third-generation and fourth-generation cephalosporins, quinolones and carbapenems. To examine the effectiveness of antibiotics, microbiological laboratory tests can be done.  However, the use of antibiotics alone is usually not enough. Surgery removing is often needed after the patient is started on antibiotics. For patients with more severe infections, the treatment should be more prudent. The method involves 48–72 hours of multiple therapies initially,  followed by a switch to a specific monotherapy.



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Centers for Disease Control and Prevention. (2012). Klebsiella pneumoniae in Healthcare Settings. https://www.cdc.gov/hai/organisms/klebsiella/klebsiella.html

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Seifi, K., Kazemian, H., Heidari, H., Rezagholizadeh, F., Saee, Y., Shirvani, F., & Houri, H. (2016). Evaluation of Biofilm Formation Among Klebsiella pneumoniae Isolates and Molecular Characterization by ERIC-PCR. Jundishapur Journal of Microbiology, 9(1), e30682. http://doi.org/10.5812/jjm.30682

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Tian, L. & Tan, R. (2016). Epidemiology of Klebsiella pneumoniae bloodstream infections in a teaching hospital: factors related to the carbapenem resistance and patient mortality. Antimicrob Resist Infect Control 5, 48


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